MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung’s Disease

BACKGROUND Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR. METHODS In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro. RESULTS Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation. CONCLUSIONS Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF.